Article | . 2017 Vol. 29, Issue. 2
Effects of Aloe-fermented Products on Improving Gastrointestinal Functions in an Inflammatory Bowel Disease Mouse Model



Hurum Central Research Institute1




2017.. 104:120


PDF XML




Aloe-fermented products (G. lucidum- aloe- fermented product: AG, H. erinaceum-aloe-fermented product: AH and P. linteus-aloe-fermented product: AP) were obtained by fermenting Ganodorma lucidum, Hericium erinaceum, and Phellinus linteus with aloe for different fermentation periods. In this study, the aloe-fermented products were examined to determine their effects on improving gastrointestinal function. Regarding inhibition of β-glucuronidase and tryptophanase, β-glucuronidase was significantly inhibited by all of the aloe-fermented products when compared to that in the control group (Natural aloe vera: AN) which showed 15.8±4.8% inhibition. When 0.5 mg and 1 mg of AG were added, regardless of the fermentation period and amount of AG used, the inhibition effect of AG was a minimum of 56%, which was very significant. Regarding inhibition of tryptophanase, the test groups with 1 mg of the aloe-fermented products exhibited better inhibition effects than the control group (AN), which showed 17.0±2.0%. When the amount of aloe-fermented product was reduced from 1 mg to 0.5 mg, the inhibition effect decreased. AG showed a maximum of 72% inhibition with four days of cultivation. To determine their effects on improving gastrointestinal function in inflammatory bowel disease, AG and AH were administered to Balb/c mice for five days after trinitrobenzene sulfonic acid solution (TNBS) injection. The test material AG, which was administered a week before colitis induction by TNBS, did not affect colitis induction. The test material AH, however, showed a slight preventive effect on colitis induction. AG and AH were also administered continuously after inducing colitis, and an alleviation of colitis was observed. Therefore, aloe-fermented products can be considered functional foods for improving gastrointestinal function, especially in case of inflammatory bowel disease.



1. Bae, H. S., Kang, S. K., Shin, I. S., Woo, S. K., Kim, Y. J., Kim, M. A., Ra, J. C. (2009) The effects of extracts mixture drink from inonotus obliquus, phellinus linteus and ganoderma lucidum on hematopoietic stem cells and lymphocyte subset of blood in human. J. Fd Hyg. Safety. 24(1):78-85.  

2. Choi, J. H., Kim, D. W., Yoo, J. K., Han, S. S., Shin, C. S. (1996) Effect of aloe on learning and memory impairment animal model SAMP8 II. feeding effect of aloe on lipid metabolism of SAMP8. Journal of life science, 6(3): 178-184.  

3. Choi, M. A., Park, N. Y., Woo, S. M., Jeong, Y. J., Shin, S. R. (2003) Characteristics of hericium erinaceus and its extracts. Korean J Food Preserv 10(4):560-564.  

4. Choi, S. S., Ha, N. J. (1999) Fecal microflora of mice in relation to diet. Korean J. microbiology, 35(2):128-132.  

5. Choi, S. W., Kim, Y. K. (2009) Studies on protective effect of bupleurum falcatum extract(SHI-1909) against experimental inflammtory bowel disease model. Journal of The Korea Academia industrial Cooperation Society. 10(3):613- 619.  

6. Cho, Y. J., An, B. J., Kim, M. U., Shim, C. S. (2006) Anti-inflammatory effect of aloe vera and aloe arborescens in phosphatidic acid-stimulated raw cells. J Korean Soc Appl Bio Chem 49(1):65-69.  

7. Han, M. J., Bae, E. A., Rhee, Y. K., Kim, D. H. (1996) Effect of mushrooms on the growth of intestinal lactic acid bacteria. Korean J Food Sci Technol 28(5):947-952.  

8. Hong, H. S., Lee, K. H., Kim, J. H., Kang, H. G., Cho, C. H., Kim, C. H. (1999) The comparative of inhibitory effect of various solvent extracts from aloe arborescens and aloe vera on tumor cell lines using clonoginic assay. Korean. J. Pharmacogn. 30(3):275-279.  

9. Hwang, K. H., Kim, H. K., Han, Y. N. (1997) Screening of inhibitory activity of edible mushrooms on dopamine β- hydroxylase. Korean J. Food Sci. Technol. 29(2):194-197.  

10. Jeong, H. Y., Kim, J. H., Hwang, S. J., Rhee, D. K. (1994) Anticancer effects of aloe on sarcoma 180 in ICR mouse and on human cancer cell lines. Yakhak Hoeji 38(3):311-321.  

11. Jeong, J. H., Wee, J. J., Shin, J. Y., Cho, J. H., Jung, D. H. (2005) Antioxidative effect of crude saponin fraction prepared from culture product of basidiomycota cultured with fresh ginseng as substrate. Korean J. Food Sci. Technol. 37(1):67-72.  

12. Joung, E. M., Hwang, I. G., Lee, H. Y., Jeong, J. H., Yu, K. W., Jeong, H. S. (2009) Changes of saponin and β- glucan content on the cultured ginseng with mushroom mycelia. J Korean Soc Food Sci Nutr 38(8):1084-1089.  

13. Jung, J. H.,  Lee, K. E., Lee, S. Y. (2006) Optimization of submerged cultivation of hericium erinaceum. KSBB Journal 21(2):96-102.  

14. Kang, M. H., Cho, S. Y., Kim, H. S., Kim, D. H., Jeong, C. S. (2005) Antigastritic and antiulcerative effect of pulmuone healthy aloe gel. Korean J. Pharm. Soc. 49(3): 237-243.  

15. Kim, D. H., Jang, I. S., Park, J. B. (1995) Protective roles of mushrooms in experimental colon carcinogenesis. Arch Pharm Res 18(2):79-83.  

16. Kim, S. H., Lee, J. N., Kim, S. H., Oh, S. J., An, S. W., Lee, J. H., Park, Y. S., Chung, E. K., Lee, H. Y. (1998) Studies on screening and comparison of biological activities from the fruiting body and mycelium of elfvingia applanata. Korean. J. Appl. Microbiol. Biotechnol. 26(4):331-337.  

17. Kim, S. W., Kim, E. S., Kim, Y. S. (1995) Studies on the polysaccharide extracted from ganoderma lucidum. J. Korean Soc. Food Nutr. 24(1):147-153.  

18. Kim, S. Y., Ryu, B. H., Park, J. W. (2008) Effects of samiunkyungtang on inflammation and fecal enzymes in ulcerative colitis animal model. J Korean Oriental Med 29(3):50-62.  

19. Kim, Y. I., Kim, B. K., Jeong, H., Lee, K. H. (1991) Production of antihypertensive constituents from ganoderma lucidumIY005 by fermentation using industrial wastes. Korean J Mycology 19(1):79-84.  

20. Lee, B. W., Lee, M. S., Park, K. M., Kim, C. H., Ahn, P. U., Choi, C. H. (1998) Anticancer activities of the extract from the mycelia of coriolus versicolor. Korean J. Appl. Microbiol. Biotechnol. 30(3):702-708.  

21. Lee, G. D., Chang, H. G., Kim, H. K. (1997) Antioxidative and nitrite-scavenging activities of edible mushrooms. Korean J. Food Sci. Technol. 29(3):432-436.  

22. Lee, K. A., Kim, M. S., Cho, H. B. (2008) Effect of extract of fermented dropwort on intestinal bacteria and enzymes in vitro. Kor J Microbiol 44(4):358-361.  

23. Lee, S. H., Woo, S. J., Koo, Y. J., Shin, H. K. (1995) Effects of mugwort, onion and polygalae radix on the intestinal environment of rats. Korean J. Food Sci. Technol. 27(4):598-604.  

24. Lee, S. Y., Kang, T. S., Moon, S. O., Lew, I. D., Lee, M. Y. (1996) Fractionation and antitumor activity of the water soluble exo-polysaccharide by submerged cultivation of ganoderma lucidum mycelium. Korean. J. Appl. Microbiol. Biotechnol. 24(4):459-464.  

25. Lee, Y. W., Roh, W. S., Kim, J. G., Kim, P. G. (1996) Effects of aloe vera on the cholesterol and vitamin D2-induced atherosclerosis in rats. J. Fd Hyg. Safety. 11(4):243-259.  

26. Oh, S. I., Lee, M. S. (2005) Antioxidative and antimutagenic effects of ganoderma lucidum krast extracts. Korean J. Food & Nutr. 18(1):54-62.  

27. Rhee, Y. K., Kim, D. H., Han, M. J. (1998) Inhibitory effect of zizyphi fructus on β-glucuronidase and tryptophanase of human intestinal bacteria. Korean J. Food Sci. Technol. 30(1):199-205.  

28. Seo, K. W., Cho, I. S., Oh, M. H., Lee, K. M., Kim, H. J. (1996) Subacute toxicity of G009, a polysaccharide isolated from ganoderma lucidum IY009. J. Fd Hyg. Safety. 11(4):261-271.  

29. Shin, S. B., Park, J. S., Kim, N. J., Kim, D. H. (1999) β-Glucuronidase-inhibitory activity and hepatoprotective effect of herbal medicines. Korean J. Pharmacogn. 30(2):111-114.  

30. Woo, N., Ahn, M. S., Lee, G. Y. (1995) Antioxidative effect of aloe (aloe arborescences) extracts on linoleic acid and soybean oil. Korean J. Soc. Food Sci. 11(5):536-541.  



online submission
online submission
online submission
online submission
Sub Menu
Sub Banner
Sub Banner
Sub Menu
Sub Banner
Sub Banner
Sub Banner
Sub Banner
Sub Menu
Sub Menu