Article | . 2018 Vol. 30, Issue. 1
Antihypertensive Effect of Peptides from Sesame, Almond, and Pumpkin Seeds: In-silico and In-vivo Evaluation



Department of Food Science and Biotechnology, College of Agriculture and Life Science, Kangwon National University1
School of Food Science and Biotechnology, Kyungpook National University2
Centre for Food Technology, Department of Biotechnology, Anna University3




2018.. 12:30


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Plant proteins are important functional ingredients in many processed food products. In particular, globular proteins from various sources play an important role in many food products. In the current study, 11S globulin protein from white sesame seeds, amandin protein from almond seeds, and cucurbitin protein from pumpkin seeds were extracted by means of ammonium sulfate precipitation and purified by anion-exchange chromatography on a DEAE-Sephadex column (20 × 30 cm). Amandin protein of almond and cucurbitin protein of pumpkin seeds both belong to the 11S globulin family. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of protein samples treated with reducing agents revealed that the isolated 11S globulin from white sesame seeds was composed of an acidic (30-33 kDa) and basic (20-24 kDa) polypeptide, amandin from almond seeds was composed of an acidic (40-42 kDa) and basic (15-20 kDa) polypeptide, and cucurbitin from pumpkin seeds was composed of an acidic (35-42 kDa) and basic (20-25 kDa) polypeptide. The alpha amylase-inhibiting activities of the three proteins was determined. Cucurbitin of pumpkin seeds had a greater alpha amylase inhibitory effect (-86%) than 11S globulin of sesame (82.6%) and amandin protein of almond seeds (76%). The antihypertensive effect of the three proteins was evaluated by a chrioallantoin membrane assay in chick embryos, which revealed that cucurbitin protein showed higher vasodilatation activity than the other two proteins.



1. Abdel-Rahim, A. A., Osman, S. A., Yasin, A. A., Abdelraheem, Y. M. (2015) Antimicrobial activity and some physiochemical properties of pumpkin (Cucurbita pepo L.) seed oil. Gezira J Eng Appl Sci 10(1):Online.   

2. Aloisi, I., Parrotta, L., Ruiz, K. B., Landi, C., Bini, L., Cai, G., Biondi, S., Del Duca, S. (2016) New insight into quinoa seed quality under salinity: changes in proteomic and amino acid profiles, phenolic content, and antioxidant activity of protein extracts. Front Plant Sci 7:656.  

3. Bedigian, D. (2010) Cultivated sesame and wild relatives in the genus Sesamum L. Sesame: The Genus Sesamum. Medicinal and Aromatic Plants-Industrial Profiles; Bedigian, D., Ed, 33-77. CRC Press: Taylor & Francis Group Boca Raton, FL.  

4. Bojórquez-Velázquez, E., Lino-López, G. J., Huerta-Ocampo, J. A., Barrera-Pacheco, A., Barba de la Rosa, A. P., Moreno, A.,  Mancilla-Margalli, N. A., Osuna-Castro, J. A. (2016) Purification and biochemical characterization of 11S globulin from chan (Hyptis suaveolens L. Poit) seeds. Food Chem 192:203-211.  

5. Brandelli, A., Daroit, D. J., Corrêa, A. P. F. (2015) Whey as a source of peptides with remarkable biological activities. Food Res In 73:149-161.  

6. Chin, K. Y., Qin, C., Cao, N., Kemp-Harper, B. K., Woodman, O. L., Ritchie, R. H. (2014) The concomitant coronary vasodilator and positive inotropic actions of the nitroxyl donor Angeli’s salt in the intact rat heart: contribution of soluble guanylyl cyclase-dependent and-independent mechanisms. Br J Pharmacol 171(7), 1722-1734.  

7. Cossenza, M., Socodato, R., Portugal, C. C., Domith, I. C., Gladulich, L. F., Encarnação, T. G., Calaza, K. C., Mendonça, H. R., Campello-Costa, P., Paes-de-Carvalho, R. (2014) Nitric oxide in the nervous system: biochemical, developmental, and neurobiological aspects. Vitam Horm 96:79-125.  

8. Da Costa, J. S. B. (2013) Development and application of molecular-based methods for the detection of tree nut allergens: the cases of almond, hazelnut and walnut. Ph.D. Thesis, Porto: Faculty of Pharmacy, University of Porto, Portugal.  

9. De Caterina, R., Libby, P., Peng, H. B., Thannickal, V. J., Rajavashisth, T. B., Gimbrone, M. A., Shin, W. S., Liao, J. K. (1995) Nitric oxide decreases cytokine-induced endothelial activation. Nitric oxide selectively reduces endothelial expression of adhesion molecules and proinflammatory cytokines. J Clin Invest 96(1):60-68.  

10. De la Cruz, S., Madrid, R., García-García, A., Alcocer, M., Martín, R., González, I., García, T. (2017) Identification and characterisation of the proteins bound by specific phage-displayed recombinant antibodies (scFv) obtained against Brazil nut and almond extracts. J Sci Food Agric 30(6):779-790.  

11. Dhillon, G. S. (2016) Protein byproducts: transformation from environmental burden into value-added products. pp. 121-144, Academic Press Elsevier, United Kingdom.  

12. Duan, X., Wu, F., Li, M., Yang, N., Wu, C., Jin, Y., Yang, J., Jin, Z., Xu, X. (2014) Naturally occurring angiotensin I-converting enzyme inhibitory peptide from a fertilized egg and its inhibitory mechanism. J Agric Food Chem 62(24):5500-5506.  

13. Ejike, C. E., Collins, S. A., Balasuriya, N., Swanson, A. K., Mason, B., Udenigwe, C. C. (2017) Prospects of microalgae proteins in producing peptide-based functional foods for promoting cardiovascular health. Trends Food Sci Technol 59:30-36.  

14. Elhardallou, S. B., Elawad, A. M., Khairi, N. A., Gobouri, A. A., Dhahawi, H. O. (2014) A review on omega-3 and omega-6 essential fatty acids: uses, benefits and their availability in pumpkins (Cucurbita maxima) seed and desert dates (Balanites aegyptiaca) seed kernel oils. Pak J Biol Sci 17(12):1195-1208.  

15. Haque, E., Chand, R. (2008) Antihypertensive and antimicrobial bioactive peptides from milk proteins. Eur Food Res Technol 227(1):7-15.  

16. Hayes, M., Tiwari, B. K. (2015) Bioactive carbohydrates and peptides in foods: an overview of sources, downstream processing steps and associated bioactivities. Int J Mol Sci 16(9):22485-22508.  

17. Hegedus, D. D., Coutu, C., Harrington, M., Hope, B., Gerbrandt, K., Nikolov, I. (2015) Multiple internal sorting determinants can contribute to the trafficking of cruciferin to protein storage vacuoles. Plant Mol Biol 88(1-2): 3-20.  

18. Hermida, R. C., Ayala, D. E., Smolensky, M. H., Fernández, J. R., Mojón, A., Portaluppi, F. (2016) Chronotherapy with conventional blood pressure medications improves management of hypertension and reduces cardiovascular and stroke risks. Hypertens Res 39(5):277-292.  

19. Hosseinian, F., Oomah, B. D., Campos-Vega, R. (Eds.) (2016) Dietary fibre functionality in food and nutraceuticals: from plant to gut. pp. 21-65, John Wiley & Sons, West Sussex, UK.  

20. Jakubczyk, A., Karaś, M., Złotek, U., Szymanowska, U. (2017) Identification of potential inhibitory peptides of enzymes involved in the metabolic syndrome obtained by simulated gastrointestinal digestion of fermented bean (Phaseolus vulgaris L.) seeds. Food Res Int 100:489-496.  

21. Jayasena, S. H. (2011) Purification and characterization of select glycoproteins of almonds (Prunus Dulcis L.).  Master Degree Thesis, Florida State University, Tallahassee, Florida.  

22. Jhong, C. H., Riyaphan, J., Lin, S. H., Chia, Y. C., Weng, C. F. (2015) Screening alpha-glucosidase and alpha-amylase inhibitors from natural compounds by molecular docking in silico. Biofactors 41(4):242-251.  

23. Kesari, P., Chaudhary, N., Sharma, A., Katiki, M., Kumar, P., Gurjar, B. R., Tomar, S., Sharma, A. K., Kumar, P. (2017) Structural, Functional and Evolutionary Aspects of Seed Globulins. Protein Pept Lett 24(3):267-277.  

24. Kumar, B. A., Lakshman, K., Nandeesh, R., Saran, G. S. (2015) Evaluation of antioxidant and anti-amylase activities of sukhasarak churna, an ayurvedic formulation. Sci Technol Arts Res J 4(2):207-210.  

25. Kumar, P., Kesari, P., Dhindwal, S., Choudhary, A. K., Katiki, M., Verma, A., Ambatipudi, K., Tomar, S., Sharma, A. K., Mishra, G., Kumar, P. (2017) A novel function for globulin in sequestering plant hormone: crystal structure of Wrightia tinctoria 11S globulin in complex with auxin. Sci Rep 7(1):4705.  

26. Kumar, R., Sharma, R., Bairwa, K., Roy, R. K., Kumar, A., Baruwa, A. (2010) Modern development in ACE inhibitors. Der Pharm Lett 2:388-419.  

27. Lee, C. H., Kim, J. H. (2014) A review on the medicinal potentials of ginseng and ginsenosides on cardiovascular diseases. J Ginseng Res 38(3):161-166.  

28. Lee, M. J., Chung, I. M., Kim, H., Jung, M. Y. (2015) High resolution LC-ESI-TOF-mass spectrometry method for fast separation, identification, and quantification of 12 isoflavones in soybeans and soybean products. Food Chem 176:254-262.  

29. López-Pedrouso, M., Bernal, J., Franco, D., Zapata, C. (2014) Evaluating two-dimensional electrophoresis profiles of the protein phaseolin as markers of genetic differentiation and seed protein quality in common bean (Phaseolus vulgaris L.). J Agric Food Chem 62(29):7200-7208.  

30. Lordan, S., Smyth, T. J., Soler-Vila, A., Stanton, C., Ross, R. P. (2013) The α-amylase and α-glucosidase inhibitory effects of Irish seaweed extracts. Food Chem 141(3):2170-2176.  

31. Mäkinen, O. (2014) Studies on quinoa (Chenopodium quinoa) for novel food and beverage applications. Ph.D. Thesis, University College Cork, Ireland.  

32. Moubarac, J. C., Parra, D. C., Cannon, G., Monteiro, C. A. (2014) Food classification systems based on food processing: significance and implications for policies and actions: a systematic literature review and assessment. Curr Obes Rep 3(2):256-272.  

33. Mutambuka, M. (2013) Iron bioavailability and consumer acceptability of extruded common bean (Phaseolus vulgaris) flour. Doctoral Dissertation, Iowa State University, Ames, USA.  

34. Natarajan, S. S., Pastor-Corrales, M. A., Khan, F. H., Garrett, W. M. (2013) Proteomic analysis of common bean (Phaseolus vulgaris L.) by two-dimensional gel electrophoresis and mass spectrometry. J. Basic Appl Sci 9:424-437.  

35. Park, K. S., Kim, Y. T., Kim, H. S., Cha, J. S., Park, K. H. (2015) Selection of the antibacterial agents for control against Pseudomonas syringae pv. syringae causing leaf spot disease on green pumpkin (Cucurbita moschata) Korean J Pestic Sci 19(2):119-124.  

36. Perez Gutierrez, R. M. (2016) Review of Cucurbita pepo (pumpkin) its phytochemistry and pharmacology. Med Chem 6:12-21.  

37. Raihana, A. N., Marikkar, J. M. N., Amin, I., Shuhaimi, M. (2015) A review on food values of selected tropical fruits’ seeds. Int J Food Prop 18(11):2380-2392.  

38. Rezig, L., Chibani, F., Chouaibi, M., Dalgalarrondo, M., Hessini, K., Guéguen, J., Hamdi, S. (2013) Pumpkin (Cucurbita maxima) seed proteins: sequential extraction processing and fraction characterization. J Agric Food Chem 61(32):7715-7721.  

39. Rosental, L., Nonogaki, H., Fait, A. (2014) Activation and regulation of primary metabolism during seed germination. Seed Sci Res 24(1):1-15.  

40. Sacan, O., Akev, N., Yanardag, R. (2017) In vitro inhibitory effect of Aloe vera (L.) Burm. f. leaf extracts on the activity of some enzymes and antioxidant activity. Indian J Biochem Biophys 54:82-89.  

41. Sathe, S. K., Wolf, W. J., Roux, K. H., Teuber, S. S., Venkatachalam, M., Sze-Tao, K. W. C. (2002) Biochemical characterization of amandin, the major storage protein in almond (Prunus dulcis L.). J Agric Food Chem 50(15):4333-4341.  

42. Scholten, E., Moschakis, T., Biliaderis, C. G. (2014) Biopolymer composites for engineering food structures to control product functionality. Food Struct 1(1):39-54.  

43. Scotter, M. J. (2011) Methods for the determination of European Union-permitted added natural colours in foods: a review. Food Addit Contam 28(5):527-596.  

44. Serna-Saldivar, S. O. (2015) Nutritional and nutraceutical features of regular and protein fortified corn tortillas. Bread and Its Fortification: Nutrition and Health Benefits. p. 322. CRC Press Taylor & Francis Group, Boca Raton, FL.  

45. Singh, A., Meena, M., Kumar, D., Dubey, A. K., Hassan, M. I. (2015) Structural and functional analysis of various globulin proteins from soy seed. Crit Rev Food Sci Nutr 55(11):1491-1502.  

46. Sweazea, K. L., Johnston, C. S., Ricklefs, K. D., Petersen, K. N. (2014) Almond supplementation in the absence of dietary advice significantly reduces C-reactive protein in subjects with type 2 diabetes. J Funct Foods 10:252-259.  

47. Te Riet, L., Van Esch, J. H., Roks, A. J., Van den Meiracker, A. H., Danser, A. J. (2015) Hypertension. Circ Res 116(6):960-975.  

48. Thomas, D. D., Heinecke, J. L., Ridnour, L. A., Cheng, R. Y., Kesarwala, A. H., Switzer, C. H., McVicar, D. W., Roberts, D. D., Glynn, S., Fukuto, J. M., Wink, D. A., Miranda, K. M. (2015) Signaling and stress: the redox landscape in NOS2 biology. Free Radic Biol Med 87:204-225.  

49. Veronezi, C. M., Jorge, N. (2015) Chemical characterization of the lipid fractions of pumpkin seeds. Nutr Food Sci 45(1):164-173.  

50. Wang, C. (2017) Growth and survival of salmonella in partially sprouted pumpkin, sunflower, and chia seeds subsequently dried for direct consumption. Doctoral Dissertation, Illinois Institute of Technology, Chicago, Illinois.  

51. Yu, Z., Yin, Y., Zhao, W., Liu, J., Chen, F. (2012) Anti-diabetic activity peptides from albumin against α-glucosidase and α-amylase. Food Chem 135(3):2078-2085.  

52. Zambrowicz, A., Pokora, M., Setner, B., Dąbrowska, A., Szołtysik, M., Babij, K., Szewczuk, Z., Trziszka, T., Lubec, G., Chrzanowska, J. (2015) Multifunctional peptides derived from an egg yolk protein hydrolysate: isolation and characterization. Amino Acids 47(2):369-380.  

53. Zhang, J. (2014) Cyclic GMP signaling in mouse retinal development and rd1 mutation induced retinal degeneration. Doctoral Dissertation, Saint Louis University, Baguio City, Philippines.  



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