Article | . 2018 Vol. 30, Issue. 1
Characterization of the Potent Mannitol Producer Leuconostoc mesenteroides KCTC13305



Department of Food Science and Biotechnology, Kangwon National University1




2018.. 41:49


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Mannitol is considered to be a generally recognized as safe (GRAS) functional food. Mannitol is approximately 70% as sweet as sucrose and can be slowly and incompletely absorbed from the intestine, supplying only about half of the energy value of glucose. The purpose of this study was to determine the optimum conditions of mannitol fermentation by Leuconostoc mesenteroides KCTC13305 isolated from kimchi. Mannitol was produced at high yield when 100 g/L fructose was used as a carbon source. The selection testing conditions were a medium with a pH of 4-8, temperature of 25-37°C, and 0 to 2% for NaCl concentration. The optimum conditions for medium pH and temperature were determined to be 6 and, 25°C, respectively, and the addition of NaCl was detrimental to both cell growth and mannitol production. Ultimately, L. mesenteroides KCTC13305 produced 56.9 ± 0.7 g/L mannitol from 100 g/L fructose in 48 h and the yield was 62.1%.



1. Aarnikunnas, J., Ronnholm, K., Palva, A. (2002) The mannitol dehydrogenase gene (mdh) from is distinct from other known bacterial mdh genes. Appl Microbiol Biotechnol 59(6):665-671.  

2. Bae, C. R., Kwon, D. Y., Cha, Y. S. (2013) Anti-obesity effects of salted and unsalted doenjang supplementation in C57BL/6J Mice Fed with High Fat Diet. Korean Soc Food Sci Nutr 42(7):1036-1042.  

3. Baer, H. H., Williams, U., Radatus, B. (1988) Nucleophilic ring-opening in a carbohydrate nitrocyclopropane: a stereospecific approach to chiral isoalkyl structures. Carbohydr Res 174:291-303.  

4. Burge, G., Saulou-Berion, C., Moussa, M., Allais, F., Athes, V., Spinnler, H. E. (2015) Relationships between the use of Embden Meyerhof pathway (EMP) or Phosphoketolase pathway (PKP) and lactate production capabilities of diverse Lactobacillus reuteri strains. J Microbiol 53(10):702-710.  

5. Duncan, D. B. (1955) Multiple range and multiple F tests. Biometrics 11:1-42.  

6. Eaves, S., Gyi, D. E., Gibb, A. G. (2016) Building healthy construction workers: Their views on health, wellbeing and better workplace design. Appl Ergon 54:10-18.  

7. Grobben, G. J., Peters, S. W., Wisselink, H. W., Weusthuis, R. A., Hoefnagel, M. H., Hugenholtz, J., Eggink, G. (2001) Spontaneous formation of a mannitol-producing variant of Leuconostoc pseudomesenteroides grown in the presence of fructose. Appl Environ Microbiol 67(6):2867-2870.  

8. Grolean, D., Chevalier, P., Tuen, T. H. (1995) Production of polyols and ethanols by the osmophilic yeast Zygo-saccharomyces rouxii. Biotechnol Lett 17:315-320.  

9. Jeong, H. J., Lee, S. G., Lee, E. J., Park, W. D., Kim, J. B., Kim, H. J. (2010) Actioxidant activity and anti-hyperglycemic activity of medicinal herbal extracts according to estraction methods. Korean J Food Sci Technol 42(5):571-577.  

10. Jung, J. Y., Lee, S. H., Lee, H. J., Seo, H. Y., Park, W.S., Jeon, C. O. (2012) Effects of starter cultures on microbial communities and metabolites during kimchi fermentation. Int J Food Microbiol 153(3):378-387.  

11. Kim, C. Y., Lee, J. H., Kim, B. H., Yoo, S. K., Seo, E. S., Cho, K. S., Day, D. F., Kim, D. (2002) Production of mannitol using NRRL B-1149. Biotechnol Bioprocess Eng 7:234-236.  

12. Kim, M. G., Oh, M. S., Jeon, J. S., Kim, H. T., Yoon, M. H. (2016) A study on antioxidant activity and antioxidant compound content by the types of tea. J Food Hyg Saf 31(2):132-139.  

13. Kim, Y. H., Kim, S. B., Kim, S. J., Park, S. W. (2016) Market and trend of alternative sweeteners. Food Sci Indust 49(3):17-28.  

14. Korakli, M., Vogel, R. F. (2003) Purification and characterisation of mannitol dehydrogenase from Lactobacillus sanfranciscensis. FEMS Microbiol Lett 220(2):281-286.  

15. Lawson, P. (2007). Mannitol. pp. 219-225. Blackwell Publishing Ltd., New Jersey, USA.  

16. Lee, H. R., Ahn, J. E., Han, N. S. (2013) Chemical mutation of for improved mannitol production: development of a high-throughput screening strategy. KSBB J 28(3):213-215.  

17. Lee, H. Y. (2013) Approval of functional ingredient of health/functional foods in Korea. Food Indust Nutri 18:1-7.  

18. Lee, J. H., Choi, H. R., Lee, S. J., Lee, M. J., Ko, Y. J., Kwon, J. W., Lee, H. K., Jeong, J. T., Lee, T. B. (2014) Blood pressure modulating effects of black raspberry extracts in vitro and in vivo. Korean J Food Sci Technol 46(3):375-383.  

19. Mechri, B., Tekaya, M., Cheheb, H., Hammami, M. (2015) Determination of mannitol sorbitol and myo-inositol in olive tree roots and rhizospheric soil by gas chromatography and effect of severe drought conditions on their profiles. J Chromatogr Sci 53(10):1631-1638.  

20. Mishra, D. K., Hwang, J. S. (2013) Selective hydrogenation of D-mannose to D-mannitol using NiO-modified TiO (NiO-TiO) supported ruthenium catalyst. Appl Catal A Gen 453:13-19.  

21. Otgonbayar, G. E., Eom, H. J., Kim, B. S., Ko, J. H., Han, N. S. (2011) Mannitol production by Leuconostoc citreum KACC 91348P isolated from Kimchi. J Microbiol Biotechnol 21(9):968-971.  

22. Park, Y. C., Oh, E. J., Jo, J. H., Jin, Y. S., Seo, J. H. (2016) Recent advances in biological production of sugar alcohols. Curr Opin Biotechnol 37:105-113.  

23. Ryu, B. H., Kim, D. H., Yun, J. W. (1996) Characteristics of mannitol production by Leuconostoc sp. KY-002. Korean J Biotechnol Bioeng 11(6):636-641.  

24. Saha, B. C., Nakamura, L. K. (2003) Production of mannitol and lactic acid by fermentation with Lactobacillus intermedius NRRL B-3693. Biotechnol Bioeng 82(7):864-871.  

25. Song, S. H., Vieille, C. (2009) Recent advances in the biological production of mannitol. Appl Microbiol Biotechnol 84(1):55-62.  

26. Vandamme, E. J., Van Loo, J., De Laporte, A. (1987) Dynamics and regulation of sucrose phosphorylase formation in fermentations. Biotechnol Bioeng 29(1):8-15.  

27. Weymarn, N., Hujanen, M., Leisola, M. (2002) Production of D-mannitol by heterofermentative lactic acid bacteria. Proc Biochem 37:1207-1213.  

28. Wisselink, H. W., Weusthuis, R. A., Eggink, G., Hugenholta, J., Grobben, G. J. (2002) Mannitol production by lactic acid bacteria. Int Dairy J 12:151-161.  

29. Wisselink, H. W., Weusthuis, R. A., Eggink, G., Hugenholta, J., Grobbrn, G. J. (2002) Mannitol production by lactic acid bacteria: a review. Inter Daity J 12:151-161.  

30. Yoo, S. K., Hur, S. S., Song, S. H., Kim, K. M., Whang, K. S. (2005) Optimization of mannitol fermentation by sp. strain JFY. J Life Sci 15(3):374-381.  

31. Yun, J. W., Kang, S. C., Ryu, B. H., Song, S. K. (1996) Production of mannitol by Lactobacillus sp. KY-107. Korean J Biotechnol Bioeng 11(3):374-379.  



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