• Research Article

    Molecular Characterization of Chilling-induced Rice RING Finger Ligase 1 (OsCRF1): Expression Pattern, In vitro Ubiquitination Assay, and Subcellular Localization
    Oh, D. G., S. Chapagain, H. Y. Cho, Y. C. Park and C. S. Jang
    Chilling (sub-optimal temperature) stress adversely affects plant growth and productivity during rice cultivation period, especially at seedling and reproductive stages. We selected ... + READ MORE
    Chilling (sub-optimal temperature) stress adversely affects plant growth and productivity during rice cultivation period, especially at seedling and reproductive stages. We selected the Oryza Sativa chilling-induced RING E3 ligase gene (OsCRF1) and examined its expression pattern in different tissues during vegetative and reproductive stages. In addition, we evaluated the phenotypes and electrolyte leakages from two rice cultivars, under chilling stress, that revealed that Donganbyeo as a chilling-insensitive type and Satbyeolbyeo as a sensitive type. qRT- PCR was employed to evaluate OsCRF1 expression patterns in both plants. Results showed that OsCTR1 is highly induced in both cultivars during chilling stress, although the patterns differ. In vitro ubiquitination assay showed clear polyubiquitin chains of OsCRF1, confirming its E3 ligase activity. Furthermore, we determined the subcellular localization of OsCRF1 through EYFP vector fusion and observed that OsCRF1 is localized within the cell nucleus. Our findings indicate that nucleus localized OsCRF1 may play a role in the modulation of responses to chilling stress. - COLLAPSE
    March 2018
  • Research Article

    Antihypertensive Effect of Peptides from Sesame, Almond, and Pumpkin Seeds: In-silico and In-vivo Evaluation
    Chelliah, R, S. R. Ramakrishnan, U. Antony, S. H. Kim, I. Khan, C. N. Tango, P. N. Kounkeu, S. Wei, M. S. Hussain, E. B. M. Daliri, R. Momna, M. Y. Kwon, E. H. Lee, H. Y. Jo, S. B. Hwang, E. J. Park, H. J. Kimand D. H. Oh
    Plant proteins are important functional ingredients in many processed food products. In particular, globular proteins from various sources play an important role ... + READ MORE
    Plant proteins are important functional ingredients in many processed food products. In particular, globular proteins from various sources play an important role in many food products. In the current study, 11S globulin protein from white sesame seeds, amandin protein from almond seeds, and cucurbitin protein from pumpkin seeds were extracted by means of ammonium sulfate precipitation and purified by anion-exchange chromatography on a DEAE-Sephadex column (20 × 30 cm). Amandin protein of almond and cucurbitin protein of pumpkin seeds both belong to the 11S globulin family. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of protein samples treated with reducing agents revealed that the isolated 11S globulin from white sesame seeds was composed of an acidic (30-33 kDa) and basic (20-24 kDa) polypeptide, amandin from almond seeds was composed of an acidic (40-42 kDa) and basic (15-20 kDa) polypeptide, and cucurbitin from pumpkin seeds was composed of an acidic (35-42 kDa) and basic (20-25 kDa) polypeptide. The alpha amylase-inhibiting activities of the three proteins was determined. Cucurbitin of pumpkin seeds had a greater alpha amylase inhibitory effect (-86%) than 11S globulin of sesame (82.6%) and amandin protein of almond seeds (76%). The antihypertensive effect of the three proteins was evaluated by a chrioallantoin membrane assay in chick embryos, which revealed that cucurbitin protein showed higher vasodilatation activity than the other two proteins. - COLLAPSE
    March 2018
  • Research Article

    Improvement of Lipid Metabolism of Inonotus obliquus and roasted Inonotus obliquus extracts in mice a fed high-fat diet

    고지방 식이 마우스에서 로스팅 유무에 따른 차가버섯의 지질대사 개선 효과

    Kim, N. E., M. O. Lee, M. H. Jang and B. H. Chung
    This study was conducted to investigate the effects of Inonotus obliquus and roasted Inonotus obliquus extract powder on lipid metabolism in C57BL/6J ... + READ MORE
    This study was conducted to investigate the effects of Inonotus obliquus and roasted Inonotus obliquus extract powder on lipid metabolism in C57BL/6J mice fed a high-fat diet (HFD). Mice were randomly divided into five groups: normal diet (ND) group, HFD group, HFD group with 1% Inonotus obliquus extract powder (HFD-I) group, HFD with roasted Inonotus obliquus extract powder condition 1 diet (HFD-RI1) group, and HFD with roasted Inonotus obliquus extract powder condition 2 diet (HFD-RI2) group. After 10 weeks, the HFD-I, HFD-RI1, and HFD-RI2 groups had a significantly reduced epididymal adipose tissue weight. Plasma triglyceride, total cholesterol, and low-density lipoprotein-cholesterol levels were significantly decreased in the HFD group compared to those of the ND group. Furthermore, the atherogenic index and cardiac risk factor were suppressed in the HFD-I, HFD-RI1, and HFD-RI2 groups, with a significant decrease detected in the HFD-RI1 group compared to the HFD group. These results suggest that roasted Inonotus obliquus extract powder may be an effective anti-obesity material by reducing plasma triglyceride and cholesterol levels. - COLLAPSE
    March 2018
  • Research Article

    Characterization of the Potent Mannitol Producer Leuconostoc mesenteroides KCTC13305

    만니톨 생산성이 우수한 Leuconostoc mesenteroides KCTC13305 균주의 생육특성

    Park, E. H., B. H. Yoo and M. D. Kim
    Mannitol is considered to be a generally recognized as safe (GRAS) functional food. Mannitol is approximately 70% as sweet as sucrose and ... + READ MORE
    Mannitol is considered to be a generally recognized as safe (GRAS) functional food. Mannitol is approximately 70% as sweet as sucrose and can be slowly and incompletely absorbed from the intestine, supplying only about half of the energy value of glucose. The purpose of this study was to determine the optimum conditions of mannitol fermentation by Leuconostoc mesenteroides KCTC13305 isolated from kimchi. Mannitol was produced at high yield when 100 g/L fructose was used as a carbon source. The selection testing conditions were a medium with a pH of 4-8, temperature of 25-37°C, and 0 to 2% for NaCl concentration. The optimum conditions for medium pH and temperature were determined to be 6 and, 25°C, respectively, and the addition of NaCl was detrimental to both cell growth and mannitol production. Ultimately, L. mesenteroides KCTC13305 produced 56.9 ± 0.7 g/L mannitol from 100 g/L fructose in 48 h and the yield was 62.1%. - COLLAPSE
    March 2018
  • Research Article

    Tolerance of Pichia farinosa KCTC27753 Isolated from Nuruk to Fermentation Inhibitors

    누룩으로부터 분리된 Pichia farinosa KCTC27753 균주의 발효저해제에 대한 내성

    Kwon, H. J., J. A. Yoon and M. D. Kim
    The production of bioethanol from lignocellulosic biomass using yeast will depend, at least in part, on the yeast strain’s tolerance to fermentation ... + READ MORE
    The production of bioethanol from lignocellulosic biomass using yeast will depend, at least in part, on the yeast strain’s tolerance to fermentation inhibitors. Pichia farinosa KCTC27753, which was isolated from nuruk in our laboratory, grows well at 46°C and pH 3.0. To explore the fermentation-inhibitor tolerance profile of this stress-tolerant strain, a cocktail composed of fermentation inhibitors released during biomass pretreatment (e.g., furfurals, phenolic compounds, and weak acids) was tested using a plate growth assay of P. farinosa KCTC27753 and control (KCTC27412, CBS7064) strains. The results of this assay showed that P. farinosa KCTC27753 had relatively higher growth rates than other strains in the presence of HMF, vanillin, and phenolic compounds. During fermentation, KCTC27753 produced 8.54 ± 0.51 g ethanol from 20 g glucose in the presence of an inhibitor cocktail (1.6%). By contrast, CBS7064 did not grow under the test conditions and strain KCTC27412 produced 6.78 ± 0.48 g ethanol from 19.6 g glucose. - COLLAPSE
    March 2018
Journal Informaiton Agriculture and Life Sciences Research Institute Journal of Agricultural, Life and Environmental Sciences
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