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2018 Vol.30, Issue 3 Preview Page

Research Article

Isolation of Asparagus Crown and Root Rot Pathogen Fusarium oxysporum and Evaluation of Fungicides for Disease Control

아스파라거스 줄기썩음병 Fusarium oxysporum의 분리 및 살균제에 대한 생장 억제율 조사

J. H. Shin1
,
J. H. Han2
,
K. S. Kim3*
신 종환1
,
한 준희2
,
김 경수3*
1Ph.D. Candidate, Division of Bioresource Sciences and Bioherb Research Institute, Kangwon National University, Chuncheon 24341, Korea
2Ph.D., Division of Bioresource Sciences and Bioherb Research Institute, Kangwon National University, Chuncheon 24341, Korea
3Professor, Division of Bioresource Sciences and Bioherb Research Institute, Kangwon National University, Chuncheon 24341, Korea
1강원대학교 생물자원과학부 한방바이오연구소 박사과정
2강원대학교 생물자원과학부 한방바이오연구소 박사
3강원대학교 생물자원과학부 한방바이오연구소 교수
* Corresponding Author
December 2018. pp. 152-160
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Abstract

Crown and root rot is a disease of asparagus caused by the fungal pathogen Fusarium oxysporum. We investigated several asparagus fields in Gangwon Province, confirmed a high incidence of crown and root rot of asparagus seedlings, and isolated pathogens from the asparagus seedlings. Based on the sequence analysis of the translation elongation factor 1, we confirmed that F. oxysporum is the most prevalent fungus causing disease in asparagus plants. We selected six fungicides (thiophanate-methyl, propiconazole, prochloraz, mancozeb, azoxystrobin, and kresoxim-methyl) and examined their effectiveness on the inhibition of mycelial growth of F. oxysporum isolates. Prochloraz was the most effective fungicide showing >90% inhibition of mycelial growth at a concentration of 0.1 µg/mL. Propiconazole was also a highly effective fungicide with approximately 97% inhibition of mycelial growth at a concentration of 10 µg/mL. Mancozeb was the least effective fungicide with <10% inhibition of mycelial growth at a concentration of 10 µg/mL and approximately 43% inhibition at a concentration of 100 µg/mL.

Keywords
Asparagus
Crown and root rot
Fungicide
Fusarium oxysporum
References
1
Blok, W. J., Bollen, G. J. (1995) Fungi on roots and stem bases of asparagus in the Netherlands: species and pathogenicity. Eur J Plant Pathol 101:15-24.
10.1007/BF01876090
2
Borrego-Benjumea, A., Melero-Vara, J., Basallote-Ureba, M. J. (2015) Organic amendments conditions on the control of Fusarium crown and root rot of asparagus caused by three Fusarium spp. Span J Agric Res 13:1-13.
10.5424/sjar/2015134-7905
3
Borrego-Benjumea, A., Basallote-Ureba, M. J., Melero-Vara, J. M., Abbasi, P. A. (2013) Characterization of Fusarium isolates from asparagus fields in southwestern Ontario and influence of soil organic amendments on Fusarium crown and root rot. Phytopathology 104:403-415.
10.1094/PHYTO-08-13-0231-R24261409
4
Cheah, L. H., Horlock, C. M., Davis, R. D. (2003) Field survey to assess spread of new asparagus diseases in Queensland. N Z Plant Prot 56:106-108.
5
Damicone, J. P., Conway, K. E., Roberts, W. (2017) Diseases of asparagus in Oklahoma. Oklahoma Cooperative Extension Fact Sheets. EPP-7646. Available at: http://pods.dasnr.okstate.edu/docushare/dsweb/Get/Document-1326/EPP-7646web.pdf.
6
Di Lenna, P., Foletto, B. (1990) Effect of nursery management on subsequent Fusarium decline of asparagus in field. Acta Hortic 271:299-304.
10.17660/ActaHortic.1990.271.43
7
Elmer, W. H. (1997) Fusarium disease of asparagus, L.W. Burgess (Ed.), Paul Nelson Memorial Symposium, APS Press, pp.248-262, St. Paul, MN.
8
Elmer, W. H. (2015) Management of Fusarium crown and root rot of asparagus. Crop Prot 73:2-6.
10.1016/j.cropro.2014.12.005
9
Falloon, P. G., Fraser, H., Nikologg, A. S. (1989) Control of Fusarium rot with thiabendazole and salt. pp.8. Final Report to New Zealand Research Council June. Crop Research Division, DSIR, Christchurch.
10
Gullino, M. L., Minuto, A., Gilardi, G., Garibaldi, A. (2002) Efficacy of azoxystrobin and other strobilurins against Fusarium wilts of carnation, cyclamen and Paris daisy. Crop Prot 21:57-61.
10.1016/S0261-2194(01)00066-7
11
Hagan, A. K., Gilliam, C. H., Fare, D. C., Bowen, K. (1991) Application rates and spray schedules of ergosterol-biosynthesis inhibitor fungicides for control of black spot of rose. Plant Dis 75:1143-1146.
10.1094/PD-75-1143
12
Hong, S. Y., Kang, M. R., Cho, E. J., Kim, H. K., Yun, S. H. (2010) Specific PCR detection of four quarantine Fusarium species in Korea. Plant Pathol J 26:409-416.
10.5423/PPJ.2010.26.4.409
13
Lacy, M. L. (1979) Effect of chemicals on stand establishment and yields of asparagus. Plant Dis Rept 63:612-616.
14
Ma, B. (2006) Azoxystrobin sensitivity and resistance management strategies of Magnaporthe grisea causing gray leaf spot on perennial ryegrass (Lolium perenne) turf. Ph.D. Thesis. Pennsylvania State University, Pennsylvania, USA.
15
Manning, W. J., Vardaro, P. M. (1977) Soil fumigation and preplant fungicide crown soaks: effects on plant growth and Fusarium incidence in newly planted asparagus. Plant Dis Rep 61:355-357.
16
Myung, Y. S. (2016) Study on occurrence and damage of diseases in minor crops. Research Report. National Institute of Agricultural Sciences, Rural Development Administration, Jeonbuk, Korea.
17
Navrozidis, I., Alexandridis, T. K., Dimitrakos, A., Lagopodi, A. L., Zalidis, G. (2018) Identification of purple spot disease on asparagus crops across spatial and spectral scales. Comput Electron Agric 148:322-329.
10.1016/j.compag.2018.03.035
18
Nel, B., Steinberg, C., Labuschagne, N., Viljoen, A. (2007) Evaluation of fungicides and sterilants for potential application in the management of Fusarium wilt of banana. Crop Prot 26:697-705.
10.1016/j.cropro.2006.06.008
19
Reid, T. C., Hausbeck, M. K., Kizilkaya, K. (2002) Use of fungicides and biological controls in the suppression of Fusarium crown and root rot of asparagus under greenhouse and growth chamber conditions. Plant Dis 86:493-498.
10.1094/PDIS.2002.86.5.493
20
Seo, H. T., Kim Y. J., Jnag, E. H., Won, J. H. (2018) Effect of the first harvest period on the timing and yield of the second harvest of asparagus (Asparagus officinalis L.) under greenhouse conditions. J Agric Life Environ Sci 30:57-63.
21
Seong, K. C., Kim, C. H., Lee, J. S., Eum, Y. C., Moon, D. K. (2012) Determination of optimum heating date for Off-season production of asparagus (Asparagus officinalis L.). J Bio-Environ Cont 21:276-280.
22
Shin, J. H., Han, J. H., Lee, J. K., Kim, K. S. (2014) Characterization of the maize stalk rot pathogens Fusarium subglutinans and F. temperatum and the effect of fungicides on mycelial growth and colony formation. Plant Pathol J 30:397-406.
10.5423/PPJ.OA.08.2014.007825506304PMC4262292
23
Singha, I. M., Kakoty, Y., Unni, B. G., Das, J., Kalita, M. C. (2016) Identification and characterization of Fusarium sp. using ITS and RAPD causing fusarium wilt of tomato isolated from Assam, North East India.
24
Son, D. M., Kim, H. J., Jung, J. M., Kim, S. J., Kim, B. S., Yun, B. K., Lee, J. H. (2016) Effect of removal period of asparagus above-ground part mother stem on the yield of spear in the following year. Trends Agric Life Sci 53:39-48.
10.29335/tals.2016.53.39
25
Song, W., Zhou, L., Yang, C., Cao, X., Zhang, L., Liu, Z. (2004) Tomato Fusarium wilt and its chemical control strategies in a hydroponic system. Crop Prot 23:243-247.
10.1016/j.cropro.2003.08.007
26
Vujanovic, V., Hamel, C., Jabaji-Hare, S., St-Arnaud, M. (2003) First report of root rot on asparagus caused by Phytophthora megasperma in Canada. Plant Dis 87:447.
10.1094/PDIS.2003.87.4.447A
27
Yin, Y., Liu, X., Li, B., Ma, Z. (2009) Characterization of sterol demethylation inhibitor-resistant isolates of Fusarium asiaticum and F. graminearum collected from wheat in China. Phytopathology 99:487-497.
10.1094/PHYTO-99-5-048719351244
28
Yoo, N. H., Jung, S. K., Lee, C. A., Choi, D. G., Yun, S. J. (2016) Post-harvest LED and UV-B irradiation enhance antioxidant properties of asparagus Spears. Hortic Sci Technol 35:188-198.
Information
  • Publisher :Agriculture and Life Sciences Research Institute, Kangwon National University
  • Publisher(Ko) :강원대학교 농업생명과학대학 농업생명과학연구원
  • Journal Title :Journal of Agricultural, Life and Environmental Sciences
  • Journal Title(Ko) :농업생명환경연구
  • Volume : 30
  • No :3
  • Pages :152-160
  • Received Date : 2018-11-02
  • Revised Date : 2018-11-14
  • Accepted Date : 2018-11-14
  • DOI :https://doi.org/10.22698/jales.20180017
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