Complete Genome Sequence and Biological Properties of <i>Cucumber mosaic virus-</i>Sp Strain in Pepper

Park, T. S., J. W. You, J. S. Park, D. J. Min and J. S. Hong

doi:10.22698/jales.20190014

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2019 Vol.31, Issue 3 Preview Page Next Page

Research Article

Complete Genome Sequence and Biological Properties of Cucumber mosaic virus-Sp Strain in Pepper Cucumber mosaic virus-Sp 계통의 전체 염기서열 및 고추에서의 생물학적 특성 분석

December 2019. pp. 114-120

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Abstract

Cucumber mosaic virus-Sp isolate (CMV-Sp) is isolated from Solanum pseudocapsicum, also called the Jerusalem Cherry. The responses of host plants to CMV-Sp were similar to CMV-Fny. CMV-Sp induced mosaic-like symptoms on Capsicum annuum ‘Bukang’, whereas, CMV-Fny did not systemically infect the plant. To analyze the nucleotide sequence of CMV-Sp, we conducted a complete genome sequencing and multiple alignment analysis, together with other CMV isolates. The results show that CMV-Sp had the highest homologies with CMV pepper isolates. In addition, the sequences of five ORFs (1a, 2a, 2b, movement protein, and coat protein) of CMV-Sp were compared to six CMV pepper isolates. The 1a, 2a, MP, and CP genes of CMV-Sp showed more than 98.3%, 98.8%, 93.2% and 96.8%, respectively identity with CMV pepper isolates at the amino acid level. The 2b protein gene of CMV-Sp showed less than 97.3% identity with other CMV pepper isolates. To confirm the genetic variation of 2b, we aligned and compared the amino acid sequences between 2b proteins. It was found that 2b protein was specifically changed from the conserved domain (²²KKQRRR²⁷) of the nuclear localization sequence 1 (NLS1) to ²²KKQKQR²⁷. Based on host response and sequence analysis, biological properties of CMV-Sp were tested on various pepper cultivars with CMV-Fny (P0 type) and CMV-CaCh1 (P1 type). CMV-Sp and CMV-CaCh1 systemically infected all pepper cultivars, in contrast to CMV-Fny. These results have led to the conclusion that genetic and biological properties of CMV-Sp are closely related to CMV pepper isolates.

Keywords

Biological property

Capsicum annuum

Complete genome sequence

Cucumber mosaic virus

Solanum pseudocapsicum

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Information

Publisher :Agriculture and Life Sciences Research Institute, Kangwon National University
Publisher(Ko) :강원대학교 농업생명과학대학 농업생명과학연구원
Journal Title :Journal of Agricultural, Life and Environmental Sciences
Journal Title(Ko) :농업생명환경연구
Volume : 31
No :3
Pages :114-120
Received Date :2019. 12. 04
Revised Date :2019. 12. 18
Accepted Date : 2019. 12. 19
DOI :https://doi.org/10.22698/jales.20190014

Journal of Agricultural, Life and Environmental Sciences ISSN:2233-8322(Print) 2508-870X(Online) 농업생명환경연구

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