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2018 Vol.30, Issue 1

Research Article

Molecular Characterization of Chilling-induced Rice RING Finger Ligase 1 (OsCRF1): Expression Pattern, In vitro Ubiquitination Assay, and Subcellular Localization
D. G. Oh1
,
S. Chapagain1
,
H. Y. Cho1
,
Y. C. Park1
,
C. S. Jang1*
,
,
,
,
1Plant Genomics Laboratory, Department of Applied Plant Sciences, Kangwon National University
*교신저자. *Corresponding Author. 
March 2018. pp. 1-11
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Abstract

Chilling (sub-optimal temperature) stress adversely affects plant growth and productivity during rice cultivation period, especially at seedling and reproductive stages. We selected the Oryza Sativa chilling-induced RING E3 ligase gene (OsCRF1) and examined its expression pattern in different tissues during vegetative and reproductive stages. In addition, we evaluated the phenotypes and electrolyte leakages from two rice cultivars, under chilling stress, that revealed that Donganbyeo as a chilling-insensitive type and Satbyeolbyeo as a sensitive type. qRT- PCR was employed to evaluate OsCRF1 expression patterns in both plants. Results showed that OsCTR1 is highly induced in both cultivars during chilling stress, although the patterns differ. In vitro ubiquitination assay showed clear polyubiquitin chains of OsCRF1, confirming its E3 ligase activity. Furthermore, we determined the subcellular localization of OsCRF1 through EYFP vector fusion and observed that OsCRF1 is localized within the cell nucleus. Our findings indicate that nucleus localized OsCRF1 may play a role in the modulation of responses to chilling stress.

Keywords
Abiotic stress
Chilling injury
Subcellular localization
Ubiquitination

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Information
  • Publisher :Agriculture and Life Sciences Research Institute, Kangwon National University
  • Publisher(Ko) :강원대학교 농업생명과학대학 농업생명과학연구원
  • Journal Title :Journal of Agricultural, Life and Environmental Sciences
  • Journal Title(Ko) :농업생명환경연구
  • Volume : 30
  • No :1
  • Pages :1-11
  • Received Date : 2017-11-18
  • Revised Date : 2017-12-28
  • Accepted Date : 2018-01-01
  • DOI :https://doi.org/10.12972/jales.20180001
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