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2018 Vol.30, Issue 1 Preview Page

Research Article

Tolerance of Pichia farinosa KCTC27753 Isolated from Nuruk to Fermentation Inhibitors

누룩으로부터 분리된 Pichia farinosa KCTC27753 균주의 발효저해제에 대한 내성

H. J. Kwon1
,
J. A. Yoon1
,
M. D. Kim1*
권 훈주1
,
윤 정아1
,
김 명동1*
1Department of Food Science and Biotechnology, Kangwon National University
1강원대학교 식품생명공학과
*교신저자. *Corresponding Author. 
March 2018. pp. 50-56
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Abstract

The production of bioethanol from lignocellulosic biomass using yeast will depend, at least in part, on the yeast strain’s tolerance to fermentation inhibitors. Pichia farinosa KCTC27753, which was isolated from nuruk in our laboratory, grows well at 46°C and pH 3.0. To explore the fermentation-inhibitor tolerance profile of this stress-tolerant strain, a cocktail composed of fermentation inhibitors released during biomass pretreatment (e.g., furfurals, phenolic compounds, and weak acids) was tested using a plate growth assay of P. farinosa KCTC27753 and control (KCTC27412, CBS7064) strains. The results of this assay showed that P. farinosa KCTC27753 had relatively higher growth rates than other strains in the presence of HMF, vanillin, and phenolic compounds. During fermentation, KCTC27753 produced 8.54 ± 0.51 g ethanol from 20 g glucose in the presence of an inhibitor cocktail (1.6%). By contrast, CBS7064 did not grow under the test conditions and strain KCTC27412 produced 6.78 ± 0.48 g ethanol from 19.6 g glucose.

Keywords
Bioethanol
Fermentation inhibitors
Nuruk
Pichia farinosa
Tolerance

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Information
  • Publisher :Agriculture and Life Sciences Research Institute, Kangwon National University
  • Publisher(Ko) :강원대학교 농업생명과학대학 농업생명과학연구원
  • Journal Title :Journal of Agricultural, Life and Environmental Sciences
  • Journal Title(Ko) :농업생명환경연구
  • Volume : 30
  • No :1
  • Pages :50-56
  • Received Date : 2018-01-20
  • Revised Date : 2018-03-17
  • Accepted Date : 2018-03-18
  • DOI :https://doi.org/10.12972/jales.20180005
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